Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and extracellular localization.Application
Western blot analysis of Fibulin-1 expression in CCD-1064Sk whole cell lysate.| Product Name | Fibulin-1 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-190 of Fibulin-1 of human origin |
| Modification Notes | Fibulin-1 is a modular glycoprotein component of the elastic extracellular matrix fibers, basement membranes and blood. Fibulin-1 self associates as well as binds to calcium, fibronectin, laminin, nidogen and fibrinogen. These interactions, individually or in combination, may account for the observed association of Fibulin-1 with basement membranes, connective tissue, elastic fibers and fibrin clots. Fibulin-1 expression is stimulated by estrogen in ovarian cancer cell lines and has been suggested as both an agent of metastasis in ovarian cancer cells and an indicator for predicting cancer risk or aggressiveness in ovarian carcinomas. Other studies point to the inhibition of cancer cell motility with increasing exposure to Fibulin-1. The exact function of Fibulin-1 in the cell is unknown. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all Fibulin-1 isoforms of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 of Fibulin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 of Fibulin-1 of human origin |
| Entrez Gene | 2192 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and extracellular localization.
Application
Western blot analysis of Fibulin-1 expression in CCD-1064Sk whole cell lysate.| Application Notes | recommended for detection of all Fibulin-1 isoforms of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
