Application
Western blot analysis of Integrin β4 expression in SW480 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tumor showing membrane and cytoplasmic staining.| Product Name | Integrin β4 Antibody (H-101) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 28-128 mapping within the extracellular domain of Integrin β4 of human origin |
| Modification Notes | Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands, such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Integrin ∫4 (ITGB4), also known as CD104, is a 1,822 amino acid single-pass type I membrane protein belonging to the Integrin ∫ chain family. Known to associate with Integrin å6, Integrin ∫4 functions as a receptor for Laminin and is predominantly expressed by epithelia. Integrin ∫4 exists as five alternatively spliced isoforms that are encoded by a gene located on human chromosome 17q25.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Integrin β4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 28-128 mapping within the extracellular domain of Integrin &beta 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 28-128 mapping within the extracellular domain of Integrin β4 of human origin |
| Entrez Gene | 3691 |
|---|
Application
Western blot analysis of Integrin β4 expression in SW480 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tumor showing membrane and cytoplasmic staining.| Application Notes | recommended for detection of Integrin β4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
