Application
Western blot analysis of METTL1 expression in HeLa whole cell lysate.Application
Western blot analysis of METTL1 expression in Hep G2 whole cell lysate.| Product Name | METTL1 Antibody (B-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of METTL1 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | METTL1 (methyltransferase like 1), also known as TRM8, is a 276 amino acid ubiquitously expressed nuclear protein belonging to the TrmB family and methyltransferase superfamily. Containing a highly conserved S-adenosylmethionine-binding domain that is typical of methyltransferases, METTL1 shares a high degree of sequence similarity with yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. METTL1 forms a complex with WDR4 and catalyzes the 7-methylguanosine modification of tRNA at position 46 (m7G46) in a widely occurring bacterial and eukaryotic process that causes the base to become positively charged. Multiple METTL1 isoforms are produced due to alternative splicing events, and the gene encoding METTL1 maps to human chromosome 12q14.1. A METTL1 pseudogene has been identified on chromosome X. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of METTL1 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of METTL1 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 4234 |
|---|
Application
Western blot analysis of METTL1 expression in HeLa whole cell lysate.
Application
Western blot analysis of METTL1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
