Application
Western blot analysis of HS3ST5 expression in MCF7 whole cell lysate.| Product Name | HS3ST5 Antibody (B-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HS3ST5 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Heparan sulfate structures, which are responsible for executing multiple biologic activities, are generated and regulated by heparan sulfate biosynthetic enzymes. HS3ST5 (heparan sulfate (glucosamine) 3-O-sulfotransferase 5), whose alternative names include 3OST5 or HS3OST5, is a 346 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus membrane and may play a role in the biosynthesis of human heparan sulfate, a blood anticoagulant. As a heparan sulfate 3-O-sulfotransferase, HS3ST5 transfers sulfate from 3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan sulfate and heparin. HS3ST5 is highly expressed in skeletal muscle and fetal brain, with lower levels found in spinal cord, cerebellum, colon and adult brain. HS3ST5 may increase susceptibility to herpes simplex virus, type 1 infection by generating an antithrombin-binding site and entry receptor for the virus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HS3ST5 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HS3ST5 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 222537 |
|---|
Application
Western blot analysis of HS3ST5 expression in MCF7 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
