Application
Western blot analysis of Occludin expression in HeLa (A), COLO 320DM (B), ECV304 (C), MDCK (D), T84 (E) and A-431 (F) whole cell lysates.Application
Western blot analysis of Occludin isoform expression in MDCK (A), COLO 320DM (B) and ECV304 (C) whole cell lysates.| Product Name | Occludin Antibody (H-279) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin |
| Entrez Gene | 4950 |
|---|
Application
Western blot analysis of Occludin expression in HeLa (A), COLO 320DM (B), ECV304 (C), MDCK (D), T84 (E) and A-431 (F) whole cell lysates.
Application
Western blot analysis of Occludin isoform expression in MDCK (A), COLO 320DM (B) and ECV304 (C) whole cell lysates.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
