Application
Western blot analysis of AChE expression in HeLa (A), Ramos (B), HuT 78 (C), PC-12 (D) and NIH/3T3 (E) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of AChE expression in 293T (A) and SW-13 (B) whole cell lysates.Application
Western blot analysis of AChE expression in non-transfected control (A) and AChE siRNA transfected (B) HeLa cells. Blot probed with AChE (H-134): . Lamin A/C (H-110): used as specificity and loading control.Application
Western blot analysis of human recombinant AChE fusion protein.| Product Name | AChE Antibody (H-134) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 481-614 mapping at the C-terminus of acetylcholinesterase (AChE) of human origin |
| Modification Notes | Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions. Alternative mRNA splicing gives rise to three forms of AChE. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic sununits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AChE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 481-614 mapping at the C-terminus of acetylcholinesterase (AChE) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 481-614 mapping at the C-terminus of acetylcholinesterase (AChE) of human origin |
| Entrez Gene | 43 |
|---|
Application
Western blot analysis of AChE expression in HeLa (A), Ramos (B), HuT 78 (C), PC-12 (D) and NIH/3T3 (E) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of AChE expression in 293T (A) and SW-13 (B) whole cell lysates.
Application
Western blot analysis of AChE expression in non-transfected control (A) and AChE siRNA transfected (B) HeLa cells. Blot probed with AChE (H-134): . Lamin A/C (H-110): used as specificity and loading control.
Application
Western blot analysis of human recombinant AChE fusion protein.| Application Notes | recommended for detection of AChE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
