Application
Western blot analysis of p-Rad9 expression in Y79 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-Rad9 Antibody (Ser 277) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 277 phosphorylated Rad9 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification | Phosphoralated |
| Modification Notes | DNA damage or incomplete replication of DNA results in the inhibition of cell cycle progression at the G1 to S or G2 to M phase checkpoints by conserved regulatory mechanisms. Chk1, Rad9 and Hus1 are involved in the signal transduction cascade that regulates cell cycle arrest at the G2 checkpoint. Chk1 functions as an essential component in the G2 phase DNA damage checkpoint, as it phosphorylates Cdc25C in response to DNA damage and thereby inhibits mitosis. Two related mammalian proteins, Hus1 and Rad9, share conserved sequence identity and function to the yeast homologs of the same names. In vivo, Rad9 is highly phosphorylated and directly associates with two other checkpoint control proteins, Rad1 and Hus1. Additionally, Rad9 associates with anti-apoptotic Bcl-2 family proteins Bcl-2 and Bcl-xL, but not with the pro-apoptotic Bax and Bad proteins. Overexpression of Rad9 induces apoptosis and indicates that Rad9 may have an additional role in regulating apoptosis after DNA damage. Human Rad9 may be phosphorylated on specific amino acid residues, including Ser 277. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 277 phosphorylated Rad9 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 277 phosphorylated Rad9 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5883 |
|---|
Application
Western blot analysis of p-Rad9 expression in Y79 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
