Application
Western blot analysis of UBE2E2 expression in PC-3 (A) and LNCaP (B) whole cell lysates.Application
Western blot analysis of UBE2E2 expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney showing cytoplasmic staining.| Product Name | UBE2E2 Antibody (S-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against UBE2E2 of human origin |
| Modification Notes | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme. The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex. The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, thereby targeting that substrate for degradation. A wide range of enzymes facilitate this proteolytic ubiquitin pathway, one of which is UBE2E2 (also known as UBCH8 in human), which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, thereby playing an important role in protein degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of UBE2E2 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against UBE2E2 of human origin |
|---|---|
| Tissue Specificity | raised against UBE2E2 of human origin |
| Entrez Gene | 7325 |
|---|
Application
Western blot analysis of UBE2E2 expression in PC-3 (A) and LNCaP (B) whole cell lysates.
Application
Western blot analysis of UBE2E2 expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney showing cytoplasmic staining.| Application Notes | recommended for detection of UBE2E2 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
