Application
Western blot analysis of BRSK2 expression in non-transfected 293T: (A), mouse BRSK2 transfected 293T: (B), HL-60 (C), K-562 (D) and IMR-32 (E) whole cell lysates.Application
Western blot analysis of BRSK2 expression in HL60 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | BRSK2 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of BRSK2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. BRSK2 (BR serine/threonine kinase 2), also known as SAD1, STK29 or PEN11B, is a 736 amino acid protein that contains one protein kinase domain and is preferentially expressed in brain and testis. One of several members of the Ser/Thr protein kinase family, BRSK2 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be involved in microtubule assembly, neuronal polarization and synaptic development. Additionally, BRSK2 may function as an autoantigen involved in small-cell lung cancer-associated limbic encephalitis. Five isoforms of BRSK2 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of BRSK2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of BRSK2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9024 |
|---|
Application
Western blot analysis of BRSK2 expression in non-transfected 293T: (A), mouse BRSK2 transfected 293T: (B), HL-60 (C), K-562 (D) and IMR-32 (E) whole cell lysates.
Application
Western blot analysis of BRSK2 expression in HL60 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
