Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Dbl expression in A-431 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Product Name | Dbl Antibody (3) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the C-terminus of Dbl of human origin |
| Modification Notes | The superfamily of GTP binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of a broad range of biological activities. One member of the family, Cdc42Hs (originally referred to as Gp or G25K), appears to represent the human homolog of the Saccharomyces cerevisiae cell division protein, Cdc42Sc. The predicted amino acid sequence of Cdc42Hs is very similar to those of various members of the Ras superfamily proteins including N-, K- and H-Ras proteins (30-35% identical), Rho proteins (50% identical) and the Rac proteins (70% identical). A second S. cerevisiae protein, Cdc24, which is known from complementation studies to act with Cdc42Sc to regulate the development of normal cell shape in yeast, contains a region of sequence homology with the Dbl oncogene product. Dbl specifically catalyzes the dissociation of GDP from Cdc42Hs, thus representing a highly selective guanine nucleotide exchange factor for Cdc42Hs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Dbl of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the C-terminus of Dbl of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the C-terminus of Dbl of human origin |
| Entrez Gene | 4168 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Dbl expression in A-431 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Application Notes | recommended for detection of Dbl of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
