Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HL-60 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed HuT 78 cells showing nuclear localization.Application
Western blot analysis of Vav expression in Jurkat (A), MOLT-4 (B), CTLL-2 (C), HL-60 (D), GM-CSF-treated K-562 (E) and CCRF-CEM (F) whole cell lysates.| Product Name | Vav Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of Vav of human origin |
| Modification Notes | The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Vav p95 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Vav of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Vav of human origin |
| Entrez Gene | 7409 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HL-60 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed HuT 78 cells showing nuclear localization.
Application
Western blot analysis of Vav expression in Jurkat (A), MOLT-4 (B), CTLL-2 (C), HL-60 (D), GM-CSF-treated K-562 (E) and CCRF-CEM (F) whole cell lysates.| Application Notes | recommended for detection of Vav p95 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
