Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane and cytoskeletal localization.Application
Western blot analysis of IQGAP2 expression in Hep G2 whole cell lysate.| Product Name | IQGAP2 Antibody (H-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 519-727 mapping to an internal region of IQGAP2 of human origin |
| Modification Notes | IQGAP1 and IQGAP2 are RasGAP-related Actin binding proteins that interact with the small GTPases Cdc42 and Rac1 and regulate cadherin-mediated cell-cell adhesion. IQGAP1 and IQGAP2 share largely related sequence similarity, and both contain a putative calponin domain, a single WW domain, four conserved IQ or calmodulin-binding domains, and a RasGAP domain. IQGAP1 binds preferentially to the GTP S-bound form of Cdc42, whereas IQGAP2 associates with both nucleotide-bound and nucleotide-free forms of Cdc42. In addition to binding Cdc42, IQGAP1 and IQGAP2 also bind Rac1, F-Actin and calmodulin. The binding of IQGAP proteins to Cdc42 and Rac1 inhibits their intrinsic and RhoGAP-stimulated GTPase activities, which thereby maintains Cdc42 and Rac1 in their active GTP-bound state. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IQGAP2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 519-727 mapping to an internal region of IQGAP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 519-727 mapping to an internal region of IQGAP2 of human origin |
| Entrez Gene | 10788 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane and cytoskeletal localization.
Application
Western blot analysis of IQGAP2 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of IQGAP2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
