Application
Western blot analysis of Rfp2 expression in non-transfected 293T: (A), human Rfp2 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.Application
Western blot analysis of Rfp2 expression in HEK293 (A), IMR-32 (B) and SK-N-MC (C) whole cell lysates and mouse embryo (D) and mouse lymph node (E) tissue extracts.| Product Name | Rfp2 Antibody (N-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of Rfp2 of human origin |
| Modification Notes | The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B box-type zinc finger, one RING finger and three zinc-binding domains. Rfp2 (Ret finger protein 2), also known as TRIM13 (tripartite motif-containing 13), CAR, RNF77 or LEU5, is a 407 amino acid protein that belongs to the TRIM protein family and contains one B box-type zinc finger and one RING-type zinc finger. Existing as two alternatively spliced isoforms designated alpha and beta, Rfp2 is thought to act as a tumor suppressor that, when defective, may be involved in the development and progression of B-cell chronic lymphocytic leukemia. Additionally, Rfp2 may function as an E3 ubiquitin ligase that is involved in protein degradation pathways related to the ER-associated degradation (ERAD) pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rfp2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of Rfp2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of Rfp2 of human origin |
| Entrez Gene | 10206 |
|---|
Application
Western blot analysis of Rfp2 expression in non-transfected 293T: (A), human Rfp2 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.
Application
Western blot analysis of Rfp2 expression in HEK293 (A), IMR-32 (B) and SK-N-MC (C) whole cell lysates and mouse embryo (D) and mouse lymph node (E) tissue extracts.| Application Notes | recommended for detection of Rfp2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
