Application
Western blot analysis of POPX2 expression in non-transfected 293T: (A), human POPX2 transfected 293T: (B) and Jurkat (C) whole cell lysates.| Product Name | POPX2 Antibody (Q-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of POPX2 of mouse and human origin by WB, IF, IHC(P), ELISA |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine protein phosphatases. POPX1 (also known as partner of PIX 1, PPM1E (protein phosphatase 1E) or PP2CH) and POPX2 (also known as partner of PIX 2, PPM1F, CaMKPase (CaM-kinase phosphatase) or FEM-2) belong to the PP2C family of serine/threonine phosphatases. Members of the PP2C family are negative regulators of cell stress response pathways. POPX2 is a ubiquitously expressed protein and POPX1 is predominantly expressed in brain and testis. POPX1 and POPX2 specifically interact with PIX (PAK interacting exchange factor) proteins and negatively regulate the activity of åPAK, a protein kinase that can lead to the breakdown of actin stress fibers and other morphological changes. POPX2 can also interact with and regulate CaMKII activity. Overexpression of POPX2 can result in caspase-dependent apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of POPX2 of mouse and human origin by WB IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of POPX2 of mouse and human origin by WB, IF, IHC(P), ELISA |
| Entrez Gene | 9647 |
|---|
Application
Western blot analysis of POPX2 expression in non-transfected 293T: (A), human POPX2 transfected 293T: (B) and Jurkat (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
