Application
Western blot analysis of POU3F4 expression in Hep G2 whole cell lysate.| Product Name | POU3F4 Antibody (K-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of POU3F4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | The Brn family of transcription factors are found in a highly restricted subset of neurons and are critical to the early embryonic development of the central nervous system. POU3F4 (POU domain, class 3, transcription factor 4), also known as OTF9, DFN3, DFNX2, BRAIN-4 or Brn-4, is a 361 amino acid class III POU domain protein that belongs to the Brn family. Localized to the nucleus, POU3F4 contains one POU-specific domain and one homeobox DNA-binding domain through which it is thought to function as a brain-specific transcription factor that effects neuronal development. Defects in the gene encoding POU3F4 are associated with X-linked deafness type 3 (DFN3), a disorder characterized by both progressive sensorineural deafness and conductive hearing loss caused by stapes gushers (large vestibular aqueducts). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of POU3F4 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of POU3F4 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 5456 |
|---|
Application
Western blot analysis of POU3F4 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
