Application
Western blot analysis of EBF2 expression in Hep G2 whole cell lysate.| Product Name | EBF2 Antibody (S-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of EBF2 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Early B-cell factor 2 (EBF2), also known as Transcription factor COE2, is a 575 amino acid protein belonging to the COE family of proteins, whose members are all helix-loop-helix transcription factors. EBF2 is a transcription factor which, in synergy with the Wnt-responsive LEF1/CTNNB1 pathway, activates the decoy receptor for RANKL, OPG, in osteoblasts. OPG, in turn, regulates osteoclast differentiation. Lack of EBF2 has been found to cause a small defect in the terminal differentiation of osteoblasts, along with reduced bone mass and an increase in osteoclasts. Localized to the nucleus, EBF2 forms a homodimer or a heterodimer with a related family member. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of EBF2 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of EBF2 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 64641 |
|---|
Application
Western blot analysis of EBF2 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
