Application
Western blot analysis of β2-Adaptin expression in non-transfected 293T: (A), human β2-Adaptin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of β2-Adaptin expression in non-transfected 293T: (A), human β2-Adaptin transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | β2-Adaptin Antibody (A-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of β2-Adaptin of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes (1). Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex (1-3). The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17 (2-4). The alpha and beta adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length (3). a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues (5,6). AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein (7). The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of &beta 2-Adaptin of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of β2-Adaptin of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 163 |
|---|
Application
Western blot analysis of β2-Adaptin expression in non-transfected 293T: (A), human β2-Adaptin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of β2-Adaptin expression in non-transfected 293T: (A), human β2-Adaptin transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
