Application
Western blot analysis of Rabex-5 expression in MIA PaCa-2 whole cell lysate.Application
Western blot analysis of Rabex-5 expression in non-transfected: (A) and human Rabex-5 transfected: (B) 293T whole cell lysates.| Product Name | Rabex-5 Antibody (H-288) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 421-708 mapping at the C-terminus of Rabex-5 of human origin |
| Modification Notes | Rabex-5 (Rab 5 GDP/GTP exchange factor), also known as RABGEF1, RAP1 or RABAPTIN-5-associated exchange factor for Rab 5, is a Rab guanine nucleotide exchange factor. Rabex-5 localizes to the cytoplasm and can associate with early endosomes. It consists of an N-terminal zinc finger domain, a GEF domain, an EET (early endosomal targeting) domain and a coiled-coil domain. The EET domain is important for the association of Rabex-5 with early endosomes and for the activation of Rab 5. Truncated Rabex-5 that is missing its EET domain can still function via an association with RABAPTIN-5. The Rabex-5/RABAPTIN-5 complex can target to early endosomes in a Rab 5-dependent manner through the binding of Rab5-GTP to RABAPTIN-5. In vitro, Rabex-5 exhibits GEF activity on its own, however, its association with RABAPTIN-5 increases its efficiency. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rabex-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 421-708 mapping at the C-terminus of Rabex-5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 421-708 mapping at the C-terminus of Rabex-5 of human origin |
| Entrez Gene | 27342 |
|---|
Application
Western blot analysis of Rabex-5 expression in MIA PaCa-2 whole cell lysate.
Application
Western blot analysis of Rabex-5 expression in non-transfected: (A) and human Rabex-5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Rabex-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
