Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing Z discs and cytoplasmic staining of myocytes.| Product Name | ADAMTS-5 Antibody (D-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of ADAMTS-5 of human origin |
| Modification Notes | ADAMTS (a disintegrin and metalloproteinase domain with Thrombospondin type 1 modules) proteins comprise a family of zinc-dependent proteases that are implicated in a variety of normal and pathological conditions, including arthritis and cancer. ADAMTS family members contain an N-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain and a C-terminus that contains a varying number of thrombospondin type 1 (TSP-1) motifs. ADAMTS-5 (A disintegrin and metalloproteinase with thrombospondin motifs 5), also known as ADAMTS11 or ADMP2, is a 930 amino acid secreted protein that localizes to the extracellular matrix and contains one disintegrin domain, one peptidase M12B domain and two TSP type-1 domains. Expressed at low levels in heart, brain, bladder, cervix and placental tissue, ADAMTS-5 uses zinc as a cofactor to catalyze the cleavage of aggregan (a cartilage proteoglycan) and is thought to be involved in aggregan turnover, specifically in the destruction of aggregan in arthritic diseases. Due to its involvement in aggregan degradation, ADAMTS-5 is thought to play a role in the pathogenesis of osteoarthritis. The ADAMTS-5 precursor is processed by furin endopeptidase to yield a smaller, active form of the expressed protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| recommended for detection of ADAMTS-5 of mouse, rat and human origin by WB, IF, IHC(P) and ELISA non cross-reactive with other ADAMTS family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of ADAMTS-5 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of ADAMTS-5 of human origin |
| Entrez Gene | 11096 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing Z discs and cytoplasmic staining of myocytes.| Application Notes | recommended for detection of ADAMTS-5 of mouse, rat and human origin by WB, IF, IHC(P) and ELISA non cross-reactive with other ADAMTS family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
