Application
Western blot analysis of phosphorylated p-Bcr expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tumor tissue extract showing cytolasmic staining.| Product Name | p-Bcr Antibody (Tyr 177) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 177 of Bcr of human origin |
| Modification | Phosphoralated |
| Modification Notes | The Bcr gene, mapping on chromosome 22, was initially identified on the basis of its fusion with the c-Abl proto-oncogene on chromosome 9 resulting in the generation of the Philadelphia chromosome in 90-95% of patients with chronic myelogenous leukemia (CML). The Bcr gene encodes for the breakpoint cluster region (Bcr) protein. A consequence of this translocation is the generation of a Bcr/c-Abl mRNA encoding an activated c-Abl protein kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, Rac 1 p21. While it has been speculated that the Bcr protein may also stimulate Rac 2 p21 GTPase activity, it has no effect on Ras p21 or Rho p21 GTPases. It is of interest that the GAP domain of Bcr maps outside of the region that remains on chromosome 22 (Philadelphia chromosome) in CML. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 177 phosphorylated Bcr of human origin, correspondingly phosphorylated Tyr 178 of mouse origin and correspondingly phosphorylated Tyr 108 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 177 of Bcr of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 177 of Bcr of human origin |
| Entrez Gene | 613 |
|---|
Application
Western blot analysis of phosphorylated p-Bcr expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tumor tissue extract showing cytolasmic staining.| Application Notes | recommended for detection of Tyr 177 phosphorylated Bcr of human origin, correspondingly phosphorylated Tyr 178 of mouse origin and correspondingly phosphorylated Tyr 108 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
