Application
Western blot analysis of CYP2J2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of CYP2J2 expression in mouse heart tissue extract.| Product Name | CYP2J2 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 243-302 mapping within an internal region of CYP2J2 of human origin |
| Modification Notes | The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP2J2 (cytochrome P450 2J2), also known as CPJ2, is a member of the cytochrome P450 protein superfamily. Localized to the ER (endoplasmic reticulum) and microsomal membranes, CYP2J2 is one of three cytochrome P450 enzymes that are responsible for metabolizing arachidonic acid to epoxyeicosatrienoic acids. Functioning via an NADPH-dependent olefin epoxidation, CYP2J2 epoxidizes endogenous cardiac arachidonic acid pools to four different isoforms of epoxyeicosatrienoic acid, all of which are important regulators of cardiovascular homeostasis and vascular tone. CYP2J2 is highly expressed in the heart with low levels of expression found in the liver, colon and kidneys. Up-regulation of CYP2J2 by a c-Jun responsive pathway is thought to promote the neoplastic phenotype of certain carcinoma cells, implicating CYP2J2 in carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYP2J2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 243-302 mapping within an internal region of CYP2J2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 243-302 mapping within an internal region of CYP2J2 of human origin |
| Entrez Gene | 1573 |
|---|
Application
Western blot analysis of CYP2J2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of CYP2J2 expression in mouse heart tissue extract.| Application Notes | recommended for detection of CYP2J2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
