Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of TIGAR expression in Jurkat (A), HeLa (B), Hep G2 (C) and Saos-2 (D) whole cell lysates.Application
Western blot analysis of TIGAR expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of TIGAR expression in Saos-2 whole cell lysate.| Product Name | TIGAR Antibody (M-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-269 mapping at the C-terminus of TIGAR of mouse origin |
| Modification Notes | TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose-2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose-2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TIGAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-269 mapping at the C-terminus of TIGAR of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-269 mapping at the C-terminus of TIGAR of mouse origin |
| Entrez Gene | 57103 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of TIGAR expression in Jurkat (A), HeLa (B), Hep G2 (C) and Saos-2 (D) whole cell lysates.
Application
Western blot analysis of TIGAR expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of TIGAR expression in Saos-2 whole cell lysate.| Application Notes | recommended for detection of TIGAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
