Application
Western blot analysis of PRPF31 expression in non-transfected: (A) and mouse PRPF31 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PRPF31 expression in non-transfected: (A) and mouse PRPF31 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PRPF31 expression in JAR (A) and JEG-3 (B) whole cell lysates and human recombinant PRPF31 (H-250) fusion protein (C).| Product Name | PRPF31 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-330 mapping within an internal region of PRPF31 of human origin |
| Modification Notes | PRPF31 (PRP31 pre-mRNA processing factor 31 homolog), also known as RP11, PRP31 or NY-BR-99, is a ubiquitously expressed protein that localizes to the nucleus and is found in Cajal bodies and speckles. PRPF31 is involved in pre-mRNA splicing and functions as a component of the U4/U6.U5 tri-snRNP (small nuclear ribonucleoprotein) complex. More specifically, PRPF31 is believed to mediate the tethering of the tri-snRNP to the spliceosome (a large ribonucleoprotien responsible for catalyzing the splicing of pre-mRNA), thereby assisting in the assembly of the mature spliceosome. Mutations in the gene encoding PRPF31 result in autosomal dominant retinitis pigmentosa type 11 (RP11), which leads to photoreceptor cell degeneration. RP11 patients initially exhibit a loss of their midperipheral visual field as well as night vision blindness. The disease eventually progresses to the loss of far peripheral visual field and finally the loss of central vision. This suggests that PRPF31 is a key player in the pre-mRNA splicing of photoreceptor-specific genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRPF31 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-330 mapping within an internal region of PRPF31 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-330 mapping within an internal region of PRPF31 of human origin |
| Entrez Gene | 26121 |
|---|
Application
Western blot analysis of PRPF31 expression in non-transfected: (A) and mouse PRPF31 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PRPF31 expression in non-transfected: (A) and mouse PRPF31 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PRPF31 expression in JAR (A) and JEG-3 (B) whole cell lysates and human recombinant PRPF31 (H-250) fusion protein (C).| Application Notes | recommended for detection of PRPF31 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
