Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of BAT1 expression in non-transfected: (A) and human BAT1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), mouse DDX39 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), mouse DDX39 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of BAT1 expression in non-transfected 293T: (A), mouse BAT1 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of BAT1 expression in non-transfected 293T: (A), mouse BAT1 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), human DDX39 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of BAT1/DDX39 expression in HS 181.Tes (A) and mouse PBL (B) whole cell lysates and K-562 (C), HeLa (D) and Jurkat (E) nuclear extracts.| Product Name | BAT1/DDX39 Antibody (H-198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-428 mapping at the C-terminus of BAT1 of human origin |
| Modification Notes | BAT1 (spliceosome RNA helicase BAT1), also known as DEAD-box protein UAP56 (56 kDa U2AF65-associated protein), HLA-B associated transcript-1 or ATP-dependent RNA helicase p47, is a member of the DECD subfamily of DEAD-box helicases. Important for mRNA splicing and nuclear export, BAT1 interacts with the mRNA export factor ALY and also functions as a splicing factor, mediating the first ATP-dependent step of spliceosome assembly. BAT1 associates with transcription elongation factor THO proteins, forming the TREX complex, and also interacts with splicing machinery to form the exon junction complex. Due to alternative splicing events, BAT1 exists in two isoforms. DDX39 (DEAD box protein 39), like BAT1, is a member of the DEAD-box family of helicases. Localized to the nucleus and expressed in lung, brain, kidney, splenn, thymus and salivary gland, DDX39 functions in a similar manner to BAT1 and is involved in pre-mRNA splicing and mRNA export out of the nucleus. DDX39 expression is upregulated in lung squamous cell carcinoma, suggesting a role for DDX39 in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BAT1 and DDX39 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-428 mapping at the C-terminus of BAT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-428 mapping at the C-terminus of BAT1 of human origin |
| Entrez Gene | 7919 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of BAT1 expression in non-transfected: (A) and human BAT1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), mouse DDX39 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), mouse DDX39 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of BAT1 expression in non-transfected 293T: (A), mouse BAT1 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of BAT1 expression in non-transfected 293T: (A), mouse BAT1 transfected 293T: (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of DDX39 expression in non-transfected 293T: (A), human DDX39 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of BAT1/DDX39 expression in HS 181.Tes (A) and mouse PBL (B) whole cell lysates and K-562 (C), HeLa (D) and Jurkat (E) nuclear extracts.| Application Notes | recommended for detection of BAT1 and DDX39 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
