Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of PBEF expression in non-transfected 293T: (A), human PBEF transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of PBEF expression in non-transfected: (A) and mouse PBEF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PBEF expression in HL-60 whole cell lysate.| Product Name | PBEF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PBEF of human origin |
| Modification Notes | Pre-B cell-enhancing factor (PBEF), also designated nicotinamide phosphoribosyltransferase (Nampt) or visfatin, belongs to the NAPRTase family of proteins. PBEF may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. It is involved in the catalysis of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide, which is important in NAD biosynthesis. This is a rate limiting step in the NAD biosynthesis pathway. Highly enriched in the visceral fat of both human and mouse, PBEF expression levels in plasma increase during the development of obesity. PBEF is a cytoplasmic protein expressed primarily in bone marrow, muscle and liver tissue, but it can also be detected in placenta, lung, kidney and heart tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Pre-B cell enhancing factor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PBEF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PBEF of human origin |
| Entrez Gene | 10135 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of PBEF expression in non-transfected 293T: (A), human PBEF transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of PBEF expression in non-transfected: (A) and mouse PBEF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PBEF expression in HL-60 whole cell lysate.| Application Notes | recommended for detection of Pre-B cell enhancing factor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
