Application
Western blot analysis of PRAME expression in non-transfected: (A) and human PRAME transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PRAME expression in non-transfected: (A) and human PRAME transfected: (B) 293T whole cell lysates.| Product Name | PRAME Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 126-205 mapping within an internal region of PRAME of human origin |
| Modification Notes | Several tumor-associated antigen families, such as MAGE, GAGE, PRAME and BAGE, are of particular interest in tumor immunology because their ex-pression, with exception of testis and fetal tissues, seems to be restricted to tumor cells. The MAGE, BAGE and GAGE genes code for distinct antigens that are recognized by autologous cytolytic T lymphocytes. Many of these antigens represent suitable targets for tumor immunotherapy, since their expression in human melanoma cells is common and highly specific. PRAME (preferentially expressed antigen of melanoma) is a melanoma antigen recognized by cytotoxic T cells (CTLs) and is expressed in a variety of cancer cells, including leukemic cells. The PRAME gene is expressed at a high level in a very large fraction of tumors, such as melanomas, non small-cell lung carcinomas, sarcomas, head and neck tumors and renal carcinomas. Therefore, PRAME is a candidate for tumor immunotherapy, even though it is expressed at low levels in certain normal tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRAME of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 126-205 mapping within an internal region of PRAME of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 126-205 mapping within an internal region of PRAME of human origin |
| Entrez Gene | 23532 |
|---|
Application
Western blot analysis of PRAME expression in non-transfected: (A) and human PRAME transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PRAME expression in non-transfected: (A) and human PRAME transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PRAME of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
