Application
Western blot analysis of AP4A Hydrolase expression in Jurkat whole cell lysate.Application
Western blot analysis of AP4A Hydrolase expression in non-transfected: (A) and human AP4A Hydrolase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AP4A Hydrolase expression in JAR (A), JEG-3 (B) and Hep G2 (C) whole cell lysates and mouse placenta (D) and mouse spleen (E) tissue extracts.| Product Name | AP4A Hydrolase Antibody (FL-147) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-147 representing full length AP4A Hydrolase of human origin |
| Modification Notes | Asymmetric diadenosine 5',5'''-P(1) P(4)-tetraphosphate (Ap4A) hydrolase is a Nudix enzyme that maintains homeostasis by using water to cleave the metabolite Ap4A symmetrically back into its original ATP and AMP molecules. Ap4A resides in pancreatic beta cells where it targets ATP-sensitive K(+) channels and depolarizes the cell membrane causing the excretion of insulin. Ap4A may be involved in the development of diabetes mellitus by raising blood glucose and lowering plasma insulin. Ap4A hydrolase is also active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, but not towards diadenosine triphosphate. Ap4A always generates ATP as one of the products. Ap4A hydrolase is involved in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AP4A Hydrolase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-147 representing full length AP4A Hydrolase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-147 representing full length AP4A Hydrolase of human origin |
| Entrez Gene | 318 |
|---|
Application
Western blot analysis of AP4A Hydrolase expression in Jurkat whole cell lysate.
Application
Western blot analysis of AP4A Hydrolase expression in non-transfected: (A) and human AP4A Hydrolase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AP4A Hydrolase expression in JAR (A), JEG-3 (B) and Hep G2 (C) whole cell lysates and mouse placenta (D) and mouse spleen (E) tissue extracts.| Application Notes | recommended for detection of AP4A Hydrolase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
