Application
Western blot analysis of eIF3α expression in MES-SA/Dx5 whole cell lysate.Application
Western blot analysis of eIF3α expression in non-transfected 293T: (A), mouse eIF3α transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of eIF3α expression in non-transfected 293T: (A), mouse eIF2α transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | eIF3α Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 9-258 mapping at the C-terminus of eIF3α of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that lead to 80S ribosomal assembly and, ultimately, translation. The eukaryotic initiation factor-3 (eIF3) scaffolding structure is the largest of the eIF complexes and includes eIF3å, eIF3∫, eIF3©, eIF3∂, eIF3e, eIF3Ω, eIF3h and eIF3q, all of which function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF3α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 9-258 mapping at the C-terminus of eIF3&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 9-258 mapping at the C-terminus of eIF3α of human origin |
| Entrez Gene | 8669 |
|---|
Application
Western blot analysis of eIF3α expression in MES-SA/Dx5 whole cell lysate.
Application
Western blot analysis of eIF3α expression in non-transfected 293T: (A), mouse eIF3α transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of eIF3α expression in non-transfected 293T: (A), mouse eIF2α transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of eIF3α of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
