Application
Western blot analysis of CARD 11 expression in non-transfected: (A) and mouse CARD 11 transfected: (B) 293T whole cell lysates.| Product Name | CARD 11 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 848-1147 mapping at the C-terminus of CARD 11 of human origin |
| Modification Notes | Modular protein interaction domains are an evolutionarily conserved protein contour feature in tertiary and quaternary protein folding that gives rise to a dynamic protein signaling network by mediating the assembly of encoded components into specific signaling complexes (1). Caspase-associated recruitment domain (CARD) proteins CARD11 and CARD14 are members of the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that function as molecular scaffolds for the assembly of multiprotein complexes at the plasma membrane (2). The human CARD11 gene maps to chromosome 7p22 and encodes a 1147 amino acid protein (3). The human CARD14 gene maps to chromosome 17q25 and encodes a 1004 amino acid protein (4). CARD11 and CARD14 can function as components of signaling pathways that lead to activation of the transcription factor NF-kappaB (5,6). The CARD domains of CARD11 and CARD14 can specifically interact with Bcl10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CARD 11 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 848-1147 mapping at the C-terminus of CARD 11 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 848-1147 mapping at the C-terminus of CARD 11 of human origin |
| Entrez Gene | 84433 |
|---|
Application
Western blot analysis of CARD 11 expression in non-transfected: (A) and mouse CARD 11 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CARD 11 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
