Application
Western blot analysis of DSC3 expression in THP-1 (A) and Jurkat (B) whole cell lysates.Application
Western blot analysis of DSC3 expression in non-transfected 293: (A), mouse DSC3 transfected 293: (B), FHs 173We (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of DSC3 expression in mouse embryo tissue extract.Application
Western blot analysis of DSC3 expression in RAW 264.7 whole cell lysate.| Product Name | DSC3 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 641-690 mapping within an extracellular domain of DSC3 of human origin |
| Modification Notes | The desmosomal cadherin desmocollin DSC1 is expressed in upper epidermis where strong adhesion is required (1). DSC1 is a type I membrane protein (4) required for strong adhesion and barrier maintenance in epidermis and contributes to epidermal differentiation (1). DSC3 is also a type I membrane protein (5) and is expressed in all living epidermal layers as well as in glandular ducts, basal matrix cells and the outer root sheath of hair follicles (3). DSC3, but not DSC1, is also present in desmosomes of the basal and suprabasal cell layers of other stratified epithelia such as cervix, tongue and esophagus as well as in cells of the basal layer of bladder urothelium and the complex epithelium of trachea (3,5). The DSC1 gene comprises 17 exons spanning approximately 33 kb on 18q12.1, and the DSC3 gene comprises 17 exons spanning approximately 49 kb on 18q12.1 (2). Alternative splicing gives rise to two DSC1 and DSC3 isoforms, designated 1A and 1B, and 3A and 3B, respectively, which differ in their carboxy termini (4,5). DSC1 and DSC3 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DSC3A and DCS3B of mouse and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 641-690 mapping within an extracellular domain of DSC3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 641-690 mapping within an extracellular domain of DSC3 of human origin |
| Entrez Gene | 1825 |
|---|
Application
Western blot analysis of DSC3 expression in THP-1 (A) and Jurkat (B) whole cell lysates.
Application
Western blot analysis of DSC3 expression in non-transfected 293: (A), mouse DSC3 transfected 293: (B), FHs 173We (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of DSC3 expression in mouse embryo tissue extract.
Application
Western blot analysis of DSC3 expression in RAW 264.7 whole cell lysate.| Application Notes | recommended for detection of DSC3A and DCS3B of mouse and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
