Application
Western blot analysis of RNase L expression of PC-3 (A) and DU 145 (B) nuclear extracts and F9 (C) and T98G (D) whole cell lysates.Application
Western blot analysis of RNase L expression of PC-3 (A) and DU 145 (B) nuclear extracts and F9 whole cell lysate (C).| Product Name | RNase L Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 442-741 mapping at the C-terminus of RNase L of human origin |
| Modification Notes | RNase L encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. Interferon treatment enhances levels of both RNase L and a group of synthetases that produce 5-prime-triphosphorylated, 2-prime,5-prime-oligoadenylates (2-5A) from ATP. The role of the 2-5A system in the control of viral and cellular growth suggests that defects in the 2-5A-dependent RNase gene could result in reduced immunity to viral infections and cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RNase L of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 442-741 mapping at the C-terminus of RNase L of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 442-741 mapping at the C-terminus of RNase L of human origin |
| Entrez Gene | 6041 |
|---|
Application
Western blot analysis of RNase L expression of PC-3 (A) and DU 145 (B) nuclear extracts and F9 (C) and T98G (D) whole cell lysates.
Application
Western blot analysis of RNase L expression of PC-3 (A) and DU 145 (B) nuclear extracts and F9 whole cell lysate (C).| Application Notes | recommended for detection of RNase L of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
