Application
Western blot analysis of ChM-1 expression in LADMAC (A) and NIH/3T3 (B) whole cell lysates.Application
Western blot analysis of ChM-1 expression in U-2 OS (A), HOS (B), SW480 (C), HEL 92.1.7 (D) and K-562 (E) whole cell lysates.Application
Western blot analysis of ChM-1 expression in NCI-H460 (A) and HL-60 (B) whole cell lysates.Application
Western blot analysis of ChM-1 expression in non-transfected: (A) and human ChM-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ChM-1 expression in human PBL whole cell lysate.| Product Name | ChM-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ChM-1 of human origin |
| Modification Notes | ChM-1 is a cartilage-specific matrix glycoprotein that stimulates the growth of chondrocytes. ChM-1 inhibits angiogenesis by disrupting the tube formation of endothelial cells and thus is responsible for the avascular nature of cartilage. ChM-1 is strongly expressed by the proliferating and hypertrophic zones in the epiphyseal plate of long bones. ChM-1 accumulates in the interterritorial matrix around the lacunae. During development, downregulation of ChM-1 permits angiogenesis and ultimately bone formation on the cartilage template. ChM-1 expression is downregulated in the presence of several growth factors including TGF∫2, FGF2 and PTHLH. ChM-1 expression may also play a role in the hypovascularity and chondroid formation of pleomorphic adenomas. The gene encoding human ChM-1 maps to chromosome 13q14-q21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ChM-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ChM-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ChM-1 of human origin |
| Entrez Gene | 11061 |
|---|
Application
Western blot analysis of ChM-1 expression in LADMAC (A) and NIH/3T3 (B) whole cell lysates.
Application
Western blot analysis of ChM-1 expression in U-2 OS (A), HOS (B), SW480 (C), HEL 92.1.7 (D) and K-562 (E) whole cell lysates.
Application
Western blot analysis of ChM-1 expression in NCI-H460 (A) and HL-60 (B) whole cell lysates.
Application
Western blot analysis of ChM-1 expression in non-transfected: (A) and human ChM-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ChM-1 expression in human PBL whole cell lysate.| Application Notes | recommended for detection of ChM-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
