Application
Western blot analysis of PHYHD1 expression in non-transfected: (A) and human PHYHD1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PHYHD1 expression in non-transfected: (A) and mouse PHYHD1 transfected: (B) 293T whole cell lysates.| Product Name | PHYHD1 Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of PHYHD1 of human origin |
| Modification Notes | PHYHD1 (phytanoyl-CoA dioxygenase domain containing 1) is a 291 amino acid protein belonging to the PHYH family and the PHYHD1 subfamily. Encoded by a gene that maps to human chromosome 9q34.11, PHYHD1 exists as three alternatively spliced isoforms and likely functions as an alpha-ketoglutatare-dependent dioxygenase. PHYHD1 participates in metal ion binding and oxidoreductase activity, thereby acting on single donors with incorporation of two atoms of oxygen. Related to PHYH (phytanoyl-CoA 2-hydroxylase), an Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase that catalyzes the initial α-oxidation step in the degradation of phytenic acid in peroxisomes, PHYHD1 similarly exhibits additional homologues in a wide range of metazoans and bacteria. PHYHD1 also may play a role in DNA methylation in early postnatal liver development and mammalian differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PHYHD1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PHYHD1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PHYHD1 of human origin |
| Entrez Gene | 254295 |
|---|
Application
Western blot analysis of PHYHD1 expression in non-transfected: (A) and human PHYHD1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PHYHD1 expression in non-transfected: (A) and mouse PHYHD1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PHYHD1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
