Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of XPC expression in Hs68 whole cell lysate.| Product Name | XPC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 641-940 mapping at the C-terminus of XPC of human origin |
| Modification Notes | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair (NER) proteins. NER provides versatile DNA repair mechanisms to en-sure the proper functioning of all cells. The majority of patients with XP carry mutations in either the XPA or XPC genes, which encode proteins involved in the recognition of damaged DNA. The gene encoding human XPC maps to chromosome 3p25. XPC forms a complex with Cen2 and the human homolog of yeast Rad23B (HR23B), both of which stabilize XPC; it also excises thymine dimers from damaged DNA. Specifically, the carboxy-terminus of XPC is re-quired for HR23B and DNA binding and, subsequently, mutations leading to carboxy-terminal truncations result in nonfunctional XPC proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of XPC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 641-940 mapping at the C-terminus of XPC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 641-940 mapping at the C-terminus of XPC of human origin |
| Entrez Gene | 7508 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of XPC expression in Hs68 whole cell lysate.| Application Notes | recommended for detection of XPC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
