Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of DLC-1 expression in non-transfected: (A) and human DLC-1 transfected: (B) 293T whole cell lysatesApplication
Western blot analysis of DLC-1 expression in c4 whole cell lysate.| Product Name | DLC-1 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 111-370 mapping within an internal region of DLC-1 of human origin |
| Modification Notes | Loss of expression of deleted in liver cancer 1 (DLC-1) protein correlates strongly with cancerous phenotype in a large number of human tissues, such as breast, liver, colon and prostate, and generally occurs due to genomic deletion or aberrant promotor methylation. The gene encoding DLC-1 maps to human chromasome 8p21-22, a region presumed to harbor tumor supressor genes based on its frequent mutation in a large number of cancers. DLC-1 localizes to the cytoplasm and restored expression leads to caspase-3 mediated apoptosis, and inhibition of cell growth and invasiveness. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Deleted in Liver Cancer 1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-370 mapping within an internal region of DLC-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-370 mapping within an internal region of DLC-1 of human origin |
| Entrez Gene | 10395 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of DLC-1 expression in non-transfected: (A) and human DLC-1 transfected: (B) 293T whole cell lysates
Application
Western blot analysis of DLC-1 expression in c4 whole cell lysate.| Application Notes | recommended for detection of Deleted in Liver Cancer 1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
