Application
Western blot analysis of XPF expression in mouse testis tissue extract.IF
Immunofluorescence of the XPF and Pt-DNA adducts in cisplatin-resistant Huh7 cells after different treatments at pH 6.5. Scale bar: 40 μm. g Quantitative analysis of the colocalization between XPF and Pt-DNA adducts.WB
Western blot analysis of XPF expression in the nucleus of cisplatin-resistant Huh7 cells after different treatments.| Product Name | XPF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer, and it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5’ incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of XPF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of XPF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of XPF of human origin |
| Entrez Gene | 2072 |
|---|
Application
Western blot analysis of XPF expression in mouse testis tissue extract.
IF
Immunofluorescence of the XPF and Pt-DNA adducts in cisplatin-resistant Huh7 cells after different treatments at pH 6.5. Scale bar: 40 μm. g Quantitative analysis of the colocalization between XPF and Pt-DNA adducts.
WB
Western blot analysis of XPF expression in the nucleus of cisplatin-resistant Huh7 cells after different treatments.| Application Notes | recommended for detection of XPF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
