Application
Western blot analysis of Rab 6 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Rab 6 expression in non-transfected: (A) and human Rab 6 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | Rab 6 Antibody (FL-208) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-208 representing full length Rab 6A of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves, at each stage, the movement of carrier vesicles; a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. Rab 6, also known as RAB6B, RAB6A or RAB6A, is a 208 amino acid protein that is ubiquitously expressed and is a member of the small GTPase superfamily. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rab 6A, 6B and 6C partially cross-reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-208 representing full length Rab 6A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-208 representing full length Rab 6A of human origin |
| Entrez Gene | 5870 |
|---|
Application
Western blot analysis of Rab 6 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Rab 6 expression in non-transfected: (A) and human Rab 6 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of Rab 6A, 6B and 6C partially cross-reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
