Application
Western blot analysis of IL-13Rα1 expression in non-transfected: (A) and human IL-13Rα1 transfected: (B) 293T whole cell lysates.| Product Name | IL-13Rα1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 128-427 mapping at the C-terminus of IL-13Rα1 of human origin |
| Modification Notes | The Th2 cytokine Interleukin-13 (IL-13) plays a critical role in allergen-induced airway hyper-responsiveness (AHR). Two different receptors exist for IL-13, designated IL-13Rå1 and 2. IL-13Rå1 exists as a heterodimer of IL-13Rå1 and IL-4Rå as a signaling subunit, whereas IL-13Rå2 acts as a decoy receptor for IL-13. Furthermore, TNFå or IL-4 stimulation induces IL-13Rå2 upregulation, while IL-13Rå1 is constitutively expressed. Cell surface localization of IL-13Rå2 abrogates IL-13 signaling, thus IL-13 induced translocation of the receptor from the cytoplasm provides a mechanism for negative-feedback of IL-13 signaling. IL-13Rå1 expression is predominant in B cells, monocytes and T cells, whereas IL-13Rå2 expression is highest in glioma cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-13Rα1 (also designated as CD213α1) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 128-427 mapping at the C-terminus of IL-13R&alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 128-427 mapping at the C-terminus of IL-13Rα1 of human origin |
| Entrez Gene | 3597 |
|---|
Application
Western blot analysis of IL-13Rα1 expression in non-transfected: (A) and human IL-13Rα1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IL-13Rα1 (also designated as CD213α1) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
