Application
Western blot analysis of Glut3 expression in U-87 MG (A) and Hep G2 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of Glut3 expression in 293T whole cell lysate.| Product Name | Glut3 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 216-265 of Glut3 of human origin |
| Modification Notes | Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. Glut1, Glut3 and Glut4 are high-affinity transporters, whereas Glut2 is a low-affinity transporter. In adipose and muscle tissue, Insulin stimulates a rapid and dramatic increase in glucose uptake, largely due to the redistribution of the Insulin-inducible glucose transporter, Glut4. In response to Insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to Insulin. Glut2 expression is seen in pancreatic b cells, hepatocytes and basolateral membranes of intestinal and epithelial cells, while the highest expression of Glut3 has been found in neuronal tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Glut3 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 216-265 of Glut3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 216-265 of Glut3 of human origin |
| Entrez Gene | 6515 |
|---|
Application
Western blot analysis of Glut3 expression in U-87 MG (A) and Hep G2 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of Glut3 expression in 293T whole cell lysate.| Application Notes | recommended for detection of Glut3 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
