Application
Western blot analysis of GS28 expression in non-transfected: (A) and mouse GS28 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GS28 expression in WEHI-231 (A) and MIA PaCa-2 (B) whole cell lysates.| Product Name | GS28 Antibody (FL-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-250 representing full length GS28 of human origin |
| Modification Notes | In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum and delivers them after covalent modification to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membranes together and may provide the energy to drive the fusion of the lipid bilayers. Golgi SNARE 27 kDa (GS27) and GS28 belong to the SNARE protein family and are important trafficking proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments. GS27 and GS28 both exist as cytoplasmically oriented integral membrane proteins. The human GS27 gene, which maps to chromosome 17q21, is located near a locus implicated in familial essential hypertension, indicating that it is a potential candidate gene for this disease. The human GS28 gene maps to chromosome 17q11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GS28 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-250 representing full length GS28 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-250 representing full length GS28 of human origin |
| Entrez Gene | 9527 |
|---|
Application
Western blot analysis of GS28 expression in non-transfected: (A) and mouse GS28 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GS28 expression in WEHI-231 (A) and MIA PaCa-2 (B) whole cell lysates.| Application Notes | recommended for detection of GS28 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
