Application
Western blot analysis of NK-3R expression in non-transfected 293T: (A), mouse NK-3R transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | NK-3R Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 361-465 mapping within a C-terminal cytoplasmic domain of NK-3R of human origin |
| Modification Notes | Substance P (SP) and neurokinin-A (NK-A) are members of the tachykinins, and they function as modulators of the immune and hematopoietic systems (2). The tachykinins interact with each of three cloned neurokinin (NK) receptors (NK-1R, NK-2R, NK-3R), with SP and NK-A exhibiting binding preferences for NK-1R and NK-2R, respectively (2). NK-4R shares close homology with NK-3R, and both have nearly identical pharmacologicl properties (1). In the normal ileum and colon, NK-1R and NK-2R are localized to smooth muscle cells of the muscularis mucosae and propria and to a few inflammatory cells of the lamina propria (3). NK-1R expression is also found in the muscular wall of submucosal blood vessels, enteric neurons and, to a lesser degree, in surface epithelial cells (3). NK-3R is found in the spinal cord in both lamina X and lamina II (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NK-3R of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 361-465 mapping within a C-terminal cytoplasmic domain of NK-3R of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 361-465 mapping within a C-terminal cytoplasmic domain of NK-3R of human origin |
| Entrez Gene | 6870 |
|---|
Application
Western blot analysis of NK-3R expression in non-transfected 293T: (A), mouse NK-3R transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of NK-3R of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
