Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of spectrin β II expression in rat brain tissue extract (A) and HeLa nuclear extract (B).| Product Name | spectrin β II Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2086-2210 mapping near the C-terminus of spectrin β II of human origin |
| Modification Notes | Spectrin is an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin å I and spectrin ∫ I are present in erythrocytes, whereas spectrin å II (also designated fodrin å) and spectrin ∫ II (also designated fodrin ∫) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ∫ II, which is involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.The human SPTBN1 gene maps to chromosome 2p21 and encodes the nonerythroid form of ∫-spectrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of spectrin β II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2086-2210 mapping near the C-terminus of spectrin &beta II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2086-2210 mapping near the C-terminus of spectrin β II of human origin |
| Entrez Gene | 6711 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of spectrin β II expression in rat brain tissue extract (A) and HeLa nuclear extract (B).| Application Notes | recommended for detection of spectrin β II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
