Application
Western blot analysis of HXK III expression in non-transfected 293T: (A), human HXK III transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HXK III expression in HeLa whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of HXK III expression in Hep G2 whole cell lysate.| Product Name | HXK III Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 mapping at the N-terminus of HXK III of human origin |
| Modification Notes | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HXK III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 mapping at the N-terminus of HXK III of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 mapping at the N-terminus of HXK III of human origin |
| Entrez Gene | 3101 |
|---|
Application
Western blot analysis of HXK III expression in non-transfected 293T: (A), human HXK III transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HXK III expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of HXK III expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of HXK III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
