Application
Western blot analysis of Separase expression in MDA-MB-231 whole cell lysates (A) and HeLa nuclear extract (B).| Product Name | Separase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1496-1795 mapping at the C-terminus of Separase of human origin |
| Modification Notes | Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, Separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, Separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active Separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Separase of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1496-1795 mapping at the C-terminus of Separase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1496-1795 mapping at the C-terminus of Separase of human origin |
| Entrez Gene | 9700 |
|---|
Application
Western blot analysis of Separase expression in MDA-MB-231 whole cell lysates (A) and HeLa nuclear extract (B).| Application Notes | recommended for detection of Separase of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
