Application
Western blot analysis of PDE4A expression in non-transfected: (A) and human PDE4A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDE4A expression in non-transfected: (A) and human PDE4A transfected: (B) 293T whole cell lysates.| Product Name | PDE4A Antibody (H-166) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 721-886 mapping at the C-terminus of PDE4A of human origin |
| Modification Notes | Phosphodiesterases (PDE) hydrolyze cAMP to 5'-AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2, while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4A gene maps to chromosome 19p13.2 and spans 50 kilobases with 17 exons. The splice variants include isoforms PDE4A1-6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDE4A isoforms 1-5 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 721-886 mapping at the C-terminus of PDE4A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 721-886 mapping at the C-terminus of PDE4A of human origin |
| Entrez Gene | 5141 |
|---|
Application
Western blot analysis of PDE4A expression in non-transfected: (A) and human PDE4A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDE4A expression in non-transfected: (A) and human PDE4A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PDE4A isoforms 1-5 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
