Application
Western blot analysis of SK3 expression in PC-12 whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Product Name | SK3 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 101-145 mapping near the N-terminus of SK3 of human origin |
| Modification Notes | Small-conductance, calcium-activated K+ channels (SK channels) are activated in a voltage-independent manner, and they have a small unit conductance and high sensitivity to calcium. SK channels 1-3 contain intracellular N- and C- termini and 6 conserved transmembrane segments. SK1 expression is restricted to the brain whereas SK2 and SK3 are more widely expressed. SK channels influence most excitable cells and participate in afterhyperpolarization (AHP) and spike-frequency adaptation. Human SK3 is a 731 amino acid protein that is expressed in muscles upon denervation, and it is a component of the presynaptic compartment in mature neuromuscular junctions. SK3 may also play a regulatory role in synaptic transmission. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-145 mapping near the N-terminus of SK3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-145 mapping near the N-terminus of SK3 of human origin |
| Entrez Gene | 3782 |
|---|
Application
Western blot analysis of SK3 expression in PC-12 whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Application Notes | recommended for detection of SK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
