Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant elafin fusion protein.| Product Name | elafin Antibody (FL-117) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-117 representing full length elafin of human origin |
| Modification Notes | Elafin, also known as elastase-specific inhibitor (ESI) or skin-derived anti-leukoproteinase (SKALP), is a low molecular weight elastase inhibitor derived from psoriatic skin. Elafin is found in the epidermis of several inflammatory skin diseases, but not in normal human epidermis. It is found in the urine of psoriatic patients, and immunohistochemical studies show that elafin is found in the suprabasal differentiated keratinocytes of psoriatic epidermis. In inflamed skin, elafin exists both as a free form and as an immobilized form covalently attached to the cornified envelopes by transglutaminase cross-linking. Although there is no allelic association between pustular psoriasis (or psoriasis in general) and polymorphism of the PI3 gene, a decrease of elafin is found in lesional skin of patients with pustular psoriasis compared with plaque-type psoriasis. The gene which encodes elafin maps to human chromosome 20q12-q13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of elafin of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-117 representing full length elafin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-117 representing full length elafin of human origin |
| Entrez Gene | 5266 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant elafin fusion protein.| Application Notes | recommended for detection of elafin of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
