Application
Western blot analysis of Laminin β-3 expression in non-transfected: (A) and human Laminin β-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Laminin β-3 expression in non-transfected: (A) and human Laminin β-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Laminin β-3 expression in HeLa whole cell lysate.Application
Western blot analysis of Laminin β-3 expression in SCC-4 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and cell surface localization.Application
Western blot analysis of human recombinant Laminin β-3 fusion protein.| Product Name | Laminin β-3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 873-1172 mapping at the C-terminus of Laminin β-3 of human origin |
| Modification Notes | The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated å, ∫ and © (1–3). A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues (4,5). During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells (6). At least eight Laminin isoforms have been described: å-1, å-2, å-3, ∫-1, ∫-2, ∫-3, ©-1 and ©-2 (7). Each isoform differs in the relative affinity with which it associates with individual Laminin receptors (7–9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Laminin β-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 873-1172 mapping at the C-terminus of Laminin &beta -3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 873-1172 mapping at the C-terminus of Laminin β-3 of human origin |
| Entrez Gene | 3914 |
|---|
Application
Western blot analysis of Laminin β-3 expression in non-transfected: (A) and human Laminin β-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Laminin β-3 expression in non-transfected: (A) and human Laminin β-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Laminin β-3 expression in HeLa whole cell lysate.
Application
Western blot analysis of Laminin β-3 expression in SCC-4 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and cell surface localization.
Application
Western blot analysis of human recombinant Laminin β-3 fusion protein.| Application Notes | recommended for detection of Laminin β-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
