Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic and membrane staining of cells in germinal center.Application
Western blot analysis of CD22 expression in BJAB whole cell lysate.| Product Name | CD22 Antibody (H-221) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 20-240 mapping at the N-terminus of CD22 of human origin |
| Modification Notes | The B lymphocyte specific CD22 antigen, also designated B-lymphocyte cell adhesion molecule (BLCAM), sialic acid-binding Ig-like lectin 2 (Siglec-2) and Leu-14, is a type I integral membrane glycoprotein, structurally similar to other cell adhesion molecules (CAMs), which acts as a regulator of B cell signaling. CD22 is expressed as both a cytoplasmic and membrane protein during discrete stages of B cell lymphocyte differentiation. The cytoplasmic form of CD22, expressed early in B cell development, is a useful marker for acute lymphocytic leukemia. The membrane form of CD22 is expressed in mature B cells prior to their differentiation into plasma cells. Alternative splicing results in two different isoforms, CD22å and CD22∫. The CD22∫ monomer is the principally occurring isoform but CD22 also appears as a heterodimer of CD22∫ and the shorter isoform, CD22å. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 20-240 mapping at the N-terminus of CD22 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 20-240 mapping at the N-terminus of CD22 of human origin |
| Entrez Gene | 933 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic and membrane staining of cells in germinal center.
Application
Western blot analysis of CD22 expression in BJAB whole cell lysate.| Application Notes | recommended for detection of CD22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
