Application
Western blot analysis of V-ATPase G1 expression in non-transfected: (A) and mouse V-ATPase G1 transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.| Product Name | V-ATPase G1 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 39-118 of V-ATPase G1 of human origin |
| Modification Notes | Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. In yeast, the V-ATPase G subunit is a soluble subunit that shares homology with the F-ATPase G subunit and may be part of a connection stalk between V1 and V0. The G2 isoform of the G subunit associates with the pore-forming a1C-subunit of L-type calcium channel and aids in proper membrane targeting of the calcium channel. The genes encoding the G1 and G2 V-ATPase subunits map to chromosomes 9q33.1 and 6p21.3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of V-ATPase G1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 39-118 of V-ATPase G1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 39-118 of V-ATPase G1 of human origin |
| Entrez Gene | 9550 |
|---|
Application
Western blot analysis of V-ATPase G1 expression in non-transfected: (A) and mouse V-ATPase G1 transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic localization.| Application Notes | recommended for detection of V-ATPase G1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
